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6 diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime 6 diamidino 2 phenylindole
    6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining/Beyo3D+DAPI+Staining+Solution/pm42908353-49-46-47
    Average 99 stars, based on 1 article reviews
    6 diamidino 2 phenylindole - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    Article Title: Transketolase-like 1 potentiates PD-1 blockade in hepatocellular carcinoma by glycolysis to prime dendritic cell lactylation.
    Article Snippet: The treated samples were then linked with either Alexa Fluorite or HRP (Beyotime, China) at room temperature. .. A combined secondary antibody was applied and left to incubate for 1 h. To maintain the integrity of the nucleus, DAPI staining (Beyotime, China) was utilized. .. Subsequent to drying, images were captured utilizing a laser scanning confocal microscope from Zeiss, Germany.


    Article Title: Transketolase-like 1 potentiates PD-1 blockade in hepatocellular carcinoma by glycolysis to prime dendritic cell lactylation
    Article Snippet: The treated samples were then linked with either Alexa Fluorite or HRP (Beyotime, China) at room temperature. .. A combined secondary antibody was applied and left to incubate for 1 h. To maintain the integrity of the nucleus, DAPI staining (Beyotime, China) was utilized. .. Subsequent to drying, images were captured utilizing a laser scanning confocal microscope from Zeiss, Germany.

    Article Title: Tu-Si-Zi-Wan reduces D-galactose-induced hepatic and cerebral oxidative damage in aging mice via the Nrf2/ARE pathway.
    Article Snippet: The samples were then sequentially incubated with primary antibodies and CoraLite® Plus 594-conjugated secondary antibodies (Proteintech Group, Wuhan, China; Cat. No. RGAM004; RRID: AB_3073502). .. After DAPI staining (Beyotime Biotechnology, Shanghai, China; Cat. No. C1006), the sections were mounted with antifade reagent and imaged via a Zeiss confocal microscope (Carl Zeiss AG, Germany) for analysis (Han et al. 2025). ..

    Article Title: Tu-Si-Zi-Wan reduces D-galactose-induced hepatic and cerebral oxidative damage in aging mice via the Nrf2/ARE pathway
    Article Snippet: The samples were then sequentially incubated with primary antibodies and CoraLite ® Plus 594-conjugated secondary antibodies (Proteintech Group, Wuhan, China; Cat. No. RGAM004; RRID: AB_3073502). .. After DAPI staining (Beyotime Biotechnology, Shanghai, China; Cat. No. C1006), the sections were mounted with antifade reagent and imaged via a Zeiss confocal microscope (Carl Zeiss AG, Germany) for analysis (Han et al. ). ..

    Imaging:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    Hybridization:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    Reverse Transcription:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    Polymerase Chain Reaction:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    Amplification:

    Article Title: Single-cell transcriptomic atlas of mouse oocyte development from growth to ovulation
    Article Snippet: .. After loading, subsequent steps followed the standard Stereo-cell workflow, including DAPI staining (Beyotime Biotechnology) and imaging for morphology and spatial registration, permeabilization and probe hybridization, on-chip reverse transcription to generate cDNA, and enzymatic cDNA release followed by bead-based cleanup and PCR amplification for downstream library preparation. ..

    other:

    Article Title: Fmoc-DDT@Fos hydrogel mitigates temporomandibular joint osteoarthritis through regulating macrophage reprogramming and ferroptosis
    Article Snippet: Samples were fixed in 4% paraformaldehyde (PFA), blocked with 5% BSA, and incubated overnight at 4 °C with primary antibodies from Abcam (rabbit anti-SYK, ab155187, 1:500; rabbit anti-CD206, ab64693, 1:500; rabbit anti-CD45, ab281586, 1:50), Thermo Fisher (rabbit anti-CD86, 942-RBM4-P1, 1:500; rabbit anti-Collagen II, PA5-99159, 1:100; rabbit anti-ACSL4, MA5-42756, 1:100; mouse anti-GPX4, 2879-MSM1-P0, 1:100), and Proteintech (mouse anti-CD80, 66406, 1:100).

    Microscopy:

    Article Title: Tu-Si-Zi-Wan reduces D-galactose-induced hepatic and cerebral oxidative damage in aging mice via the Nrf2/ARE pathway.
    Article Snippet: The samples were then sequentially incubated with primary antibodies and CoraLite® Plus 594-conjugated secondary antibodies (Proteintech Group, Wuhan, China; Cat. No. RGAM004; RRID: AB_3073502). .. After DAPI staining (Beyotime Biotechnology, Shanghai, China; Cat. No. C1006), the sections were mounted with antifade reagent and imaged via a Zeiss confocal microscope (Carl Zeiss AG, Germany) for analysis (Han et al. 2025). ..

    Article Title: Tu-Si-Zi-Wan reduces D-galactose-induced hepatic and cerebral oxidative damage in aging mice via the Nrf2/ARE pathway
    Article Snippet: The samples were then sequentially incubated with primary antibodies and CoraLite ® Plus 594-conjugated secondary antibodies (Proteintech Group, Wuhan, China; Cat. No. RGAM004; RRID: AB_3073502). .. After DAPI staining (Beyotime Biotechnology, Shanghai, China; Cat. No. C1006), the sections were mounted with antifade reagent and imaged via a Zeiss confocal microscope (Carl Zeiss AG, Germany) for analysis (Han et al. ). ..



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing