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Dapi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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staining with dapi  (Vector Laboratories)
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of <t>DAPI-stained</t> cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number <t>of</t> <t>adherent</t> cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.
Staining With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear staining with dapi  (Vector Laboratories)
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of <t>DAPI-stained</t> cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number <t>of</t> <t>adherent</t> cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.
Nuclear Staining With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Boster Bio)
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of <t>DAPI-stained</t> cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number <t>of</t> <t>adherent</t> cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.
Dapi, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 6 diamindin 2 phenylindol dapi miltenyi biotec  (Miltenyi Biotec)
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of <t>DAPI-stained</t> cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number <t>of</t> <t>adherent</t> cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.
4 6 Diamindin 2 Phenylindol Dapi Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi staining solution  (Vector Laboratories)
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of <t>DAPI-stained</t> cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number <t>of</t> <t>adherent</t> cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.
Dapi Staining Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of DAPI-stained cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number of adherent cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.

Journal: Bioengineering

Article Title: Beyond Decellularization: Remnant Mitochondrial DNA Can Act as Hidden Damage-Associated Molecular Pattern

doi: 10.3390/bioengineering13020193

Figure Lengend Snippet: HpaII treatment of human liver scaffolds reduced macrophage activation and proliferation. Representative images of DAPI-stained cell nuclei of THP-cells on untreated ( A ) and HpaII-treated ( B ) liver ECM, and number of adherent cells per mm 2 . Scalebars: 100 µm. ( C ) Proliferation of M0 macrophages after 4 days of culture with decellularized scaffold, either untreated ( n = 3) ( D ) or treated with HpaII ( n = 3) ( E ). Representative images are shown. Cell nuclei (blue), Ki-67 (red), and F-actin (Phalloidin, green); scalebars: 200 µm. More ki-67 positive cells were detected in cultures with untreated scaffolds compared to HpaII-treated scaffolds. ( F ) Number of Ki-67 + cells per mm 2 plotted as min to max bars with line at mean. ( G – I ) Cytokine production by M0 macrophages after 4 days of culture with decellularized scaffolds. TNF-α, IL-1β, and IL-6 cytokine production was determined using ELISA. Untreated scaffolds ( n = 6) induced production of all cytokines. The HpaII treatment of scaffolds ( n = 6) significantly reduced TNF-α and IL-1β production. * p < 0.05 and ** p < 0.01.

Article Snippet: Adherent THP-1-cells were assessed by staining with DAPI (Vectashield antifade mounting medium with DAPI, Vector Laboratories Inc., Neward, CA, USA) and were visualized with confocal microscopy (Leica SP8 DLS Lightsheet microscope, Wetzlar, Germany) at 20× magnification.

Techniques: Activation Assay, Staining, Enzyme-linked Immunosorbent Assay